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Vagina wanita usia reproduktif terdapat flora normal yaitu Candida albicans menyebabkan >90% kasus vaginitis simtomatik. Candida albicans sering menimbulkan kandidiasis vaginistis pada wanita dengan gejala utama flour albus yang sering disertai rasa gatal. Penggunaan air toilet yang tercemar Candida sp menyebabkan infeksi ini sering terjadi, sedangkan oleh siswi/pengguna toilet digunakan untuk membersihkan diri. Infeksi ini dapat juga terjadi akibat kontaminasi silang di sekitar toilet ataupun lingkungan sekitar. Salah satu toilet yang merupakan tempat umum adalah toilet sekolah, sehingga penting kiranya dilakukan penelitian terhadap toilet sekolah tersebut. Penelitian dilakukan di SMA Negeri 48 Jakarta Timur, rancangan penelitian menggunakan Cross sectional Study, sampel adalah seluruh air bak penampungan toilet siswi. Pengujian sampel menggunakan media Saboraud Dextrosa Agar (SDA) dengan pewarnaan Lacto Phenol Cotton Blue (LPCB). Hasil pengamatan didapatkan 7 bak penampung air di toilet terkontaminasi jamur Candida albicans dan tidak ada perbedaan waktu pengambilan sampel air (pagi dan sore) terhadap kontaminasi jamur Candida albicans. Berdasarkan penelitian disimpulkan terdapat pertumbuhan jamur Candida sp pada air bak toilet sebanyak 7 (70%) sampel. Disarankan pengurasan bak toilet secara teratur minimal 1-2 hari sekali, membersihkan lantai toilet, melakukan pembersihan toilet secara rutin dengan pengurasan bak menggunakan cairan pembersih atau membersihkan bak toilet saja

The vagina of women of reproductive age has normal flora, namely Candida albicans, causing >90% of cases of symptomatic vaginitis. Candida albicans often causes candidiasis vaginitis in women with the main symptom being flour albus which is often accompanied by itching. The use of toilet water contaminated with Candida sp causes this infection to occur frequently, while female students/toilet users use it for self-cleaning. This infection can also occur due to cross-contamination around the toilet or the surrounding environment. One of the toilets which is a public place is the school toilet, so it is important to do research on the school toilet. The research was conducted at SMA Negeri 48 East Jakarta, the research design used a cross-sectional study, the samples were all the water in the female toilet tanks. Sample testing used Saboraud Dextrose Agar (SDA) medium stained with Lacto Phenol Cotton Blue (LPCB). The observation results showed that 7 water reservoirs in the toilet were contaminated with Candida albicans fungus and there was no difference in the time of taking water samples (morning and evening) for Candida albicans contamination. Based on the research, it was concluded that there was growth of Candida sp fungus in the toilet tub water in 7 (70%) samples. It is recommended to drain the toilet tub regularly at least once every 1-2 days, clean the toilet floor, carry out routine toilet cleaning by draining the tub using cleaning fluid or just cleaning the toilet tub